How do you prepare sodium chloride 150 mM?

To prepare 150 mM sodium chloride (NaCl) solution, follow these steps:

1. Calculate the amount of NaCl needed:

The concentration of NaCl is 150 mM (millimolar), which means 150 millimoles of NaCl per liter of solution. The molecular weight of NaCl is approximately 58.44 g/mol.

Amount of NaCl = Concentration (in mM) x Molecular weight (g/mol) x Volume (in L)

Amount of NaCl = 150 mM x 58.44 g/mol x 1 L

Amount of NaCl ≈ 8.766 g

2. Weigh the required amount of NaCl:

Using an analytical balance, weigh 8.766 grams of NaCl.

3. Dissolve NaCl in a small volume of deionized water:

Transfer the weighed NaCl to a clean beaker or flask. Add a small amount of deionized water, just enough to dissolve the NaCl. Stir or swirl the solution until all the NaCl is dissolved.

4. Transfer the solution to a volumetric flask:

Choose a volumetric flask with a volume of 1 liter (1000 mL). Transfer the NaCl solution into the volumetric flask using a graduated cylinder or pipette.

5. Add more deionized water to reach the calibration mark:

Slowly add deionized water to the volumetric flask until the liquid level reaches the calibration mark. Swirl the flask gently to ensure thorough mixing.

6. Mix thoroughly:

Once the solution reaches the calibration mark, stopper the volumetric flask and invert it several times to ensure uniform mixing.

Your 150 mM sodium chloride solution is now ready to use. Remember to label the bottle or container appropriately, including the concentration, date prepared, and any other relevant information.