What use is test in identifying an unknown sugar?
Benedict's test: This test is used to identify the presence of reducing sugars, which are sugars that can react with Benedict's reagent to form a colored product. Reducing sugars include glucose, fructose, and lactose. To perform Benedict's test, a small amount of the sugar sample is added to a test tube containing Benedict's reagent. The test tube is then heated, and the color of the solution is observed. If the solution turns green, yellow, or orange, the sugar sample contains a reducing sugar. If the solution remains blue, the sugar sample does not contain a reducing sugar.
Seliwanoff's test: This test is used to distinguish between ketohexoses and aldohexoses. Ketohexoses are sugars that have a keto group (C=O) on the second carbon atom, while aldohexoses are sugars that have an aldehyde group (CHO) on the first carbon atom. To perform Seliwanoff's test, a small amount of the sugar sample is added to a test tube containing Seliwanoff's reagent. The test tube is then heated, and the color of the solution is observed. If the solution turns red, the sugar sample is a ketohexose. If the solution remains colorless, the sugar sample is an aldohexose.
Molisch's test: This test is used to identify the presence of carbohydrates. To perform Molisch's test, a small amount of the sugar sample is added to a test tube containing Molisch's reagent. The test tube is then heated, and the color of the solution is observed. If the solution turns purple, the sugar sample contains a carbohydrate.
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