Why is each of these precautions necessary passing inoculating loops through a flame sealing the lid petri dish with tape incubating at maximum temperature 25 celsius?

Passing inoculating loops through a flame: This step is necessary to sterilize the inoculating loop and prevent contamination of the culture. The loop is heated until it glows red-hot, which kills any microorganisms that may be present on it.

Sealing the lid of petri dish with tape: This step helps to prevent evaporation of the culture medium and reduces the risk of contamination. The tape creates a barrier between the culture and the outside environment, preventing microorganisms from entering the dish.

Incubating at maximum temperature 25 degrees Celsius: Most bacteria grow best at temperatures around 37 degrees Celsius. However, some bacteria, such as those that cause food poisoning, can grow at temperatures as low as 25 degrees Celsius. Incubating the culture at a lower temperature helps to slow the growth of these bacteria and reduces the risk of contamination.