What uv spectrum peak for caffeine from tea?

Caffeine, a purine alkaloid, exhibits characteristic absorption peaks in the ultraviolet (UV) spectrum when extracted from tea or other sources. The primary UV absorption peak for caffeine is generally observed around 275 nm. This peak corresponds to the electronic transitions within the caffeine molecule, specifically the π→π* transitions of the aromatic rings.

When analyzing caffeine from tea, the UV spectrum can provide useful information for qualitative identification and quantification of caffeine content. The absorbance at the 275 nm peak is typically measured and compared with known caffeine standards to determine the caffeine concentration in the tea sample.

It's important to note that the specific UV spectrum of caffeine may vary slightly depending on the extraction method, solvent used, and potential interference from other compounds in the tea sample. Therefore, proper sample preparation and appropriate analytical techniques are necessary to accurately measure the caffeine content using UV spectroscopy.